The Orchid Forum, for the UK and Europe (previously known as The UK Orchid Forum) • View topic - I have germinated some seed-update 26-11-2014
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PostPosted: Mon Jun 08, 2009 2:24 pm 
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I have managed, at my third attempt, to get germination of some cattleya seed sown at home in the kitchen. It seems odd that such small green dots inside a glass jar could make me feel that I have achieved something (but they do :D)

I have been looking carefully for quite a few days now to check for signs of germination. When I asked the wife to verify a green tinge in the jar she said it was wishfull thinking, but even she agrees now there is something growing. :D

Still lots of hurdles to overcome to get a viable plant, but I am one step nearer than I was a few weeks ago.


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Last edited by cricketerry on Wed Nov 26, 2014 11:24 am, edited 21 times in total.

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PostPosted: Mon Jun 08, 2009 3:10 pm 
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How very exciting Terry! :D :D Well done so far. 8-)

I'll be keeping my fingers crossed for you that all continues smoothly and without problems.

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PostPosted: Mon Jun 08, 2009 3:14 pm 
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oooh nice! Can't say it looks all that attractive at the moment :lol:

Who knows, maybe we'll have a beautiful hybrid flowering in about 5 years time called Cat. TerryUkOrchidForum


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PostPosted: Mon Jun 08, 2009 3:18 pm 
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Thats fantastic Terry!

:D
Well done ! I know you will be feeling a great satisfaction! 8-)

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PostPosted: Mon Jun 08, 2009 3:21 pm 
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good on you Terry
i find runner beans easier :lol:


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PostPosted: Mon Jun 08, 2009 3:33 pm 
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tom wrote:
i find runner beans easier :lol:

Now I would normally agree Tom but between the chickens (that escaped) and the pigeons I have planted 3 lots of beans so far this year.

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PostPosted: Mon Jun 08, 2009 4:18 pm 
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Lucky you pidgeon omelette
again well done this is just the start best of luck


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PostPosted: Mon Jun 08, 2009 4:28 pm 
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They look absolutely great Terry - keep them going!

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PostPosted: Mon Jun 08, 2009 5:47 pm 
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Many congratulations Terry, I admire your patience (which I don't have) and its been justly rewarded.
I still have my aerangis pods on the plant, Theo reckoned they might be ready around now.
You Post may have just inspired me to have a look and do something with them (although with a view not to expect much/anything so I won't be disappointed)

Well done!

stevek


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PostPosted: Mon Jun 08, 2009 8:08 pm 
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Terry,

Congratulations! As you say a long way to go but you have got this far which is far further then I will probably ever get! :lol:

Where do we place orders? ;) :lol: :lol: :lol:

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PostPosted: Mon Jun 08, 2009 9:04 pm 
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stevek wrote:
Many congratulations Terry, I admire your patience (which I don't have) and its been justly rewarded.
I still have my aerangis pods on the plant, Theo reckoned they might be ready around now.
You Post may have just inspired me to have a look and do something with them (although with a view not to expect much/anything so I won't be disappointed)

Well done!

stevek



any results?
flasks are prepared for them here ...

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PostPosted: Mon Jun 08, 2009 9:05 pm 
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congratulations, Terry, I always mentioned sowing isnĀ“t difficult and IĀ“m proud you tried and succeeded in finally.
bye
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PostPosted: Mon Jun 08, 2009 9:12 pm 
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P_Koz wrote:

Where do we place orders? ;) :lol: :lol: :lol:


Paul place them here in about 2016 :lol:

On a more serious note I know I can raise plants from flasks produced by others because I have done it to flowering and am still doing it with as yet unflowered plants. That is the easier part. The two bits I have not done is sowing, germinating seed then growing it on in the mother flask and finally (and I suspect the most difficult part) will be transplanting out of the mother flask without the aid of a Laminar Flow cabinet or similar. I have visions of beautiful molds growing in the flasks. :lol:

Even now I have germination there is plenty that can still go wrong. It just means I can tick one more little bit off the list of things to do.

Theo please remember that you are my back-up for when things go wrong ;)

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PostPosted: Mon Jun 08, 2009 9:34 pm 
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Theo wrote:
any results?
flasks are prepared for them here ...

Many thanks for your kind offer Theo but I won't take this first attempt too seriously and I'm sure you have more than enough seeds to deal with without my humble little efforts?

stevek


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PostPosted: Tue Jun 09, 2009 11:42 am 
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Terry,

Most impressed! I looked at doing i for Hardys and just reading what was involved left me speachless.

Maybe one day ...

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PostPosted: Tue Jun 09, 2009 12:48 pm 
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Congratulation Terry, I'm very impressed. I would love to have a go, but have always been put off by all the pitfalls.


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PostPosted: Tue Jun 09, 2009 1:49 pm 
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Well done Terry! You seem to have gotten much better germination that I.

Car to share your technique?

Keep us posted.

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PostPosted: Tue Jun 09, 2009 4:50 pm 
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Tyrone I can tell you how I did it but this is no recommendation, bear in mind that this is my first successful attempt having failed twice before.

My previous failures were one of sterile conditions but no germination and a second of non sterile conditions (mould growth) and no germination.

Variables I did not know before sowing were if the seed was viable, had I sterilized it properly, did I introduce spores when I opened the flasks to sow the seed. In fact all sorts of things. The one thing I was sure of was that the virgin flasks were sterile as I had made them up several weeks prior to sowing and there was no sign of mould growth.

For this last attempt I knew there were some viable seed as Theo had tested it, so one possible problem was eliminated. I sterilized the seed for 18 mins in a 3% solution of Hydrogen Peroxide (H2O2) with a drop of washing up liquid. I did this in a small sample bottle that had an inverted conical base to it. This base let the seed collect in it so that I could suck up a little with a plastic dropper.

I put 2 large saucepans of water on the hob and let them boil away to create a wall of steam, opened the flasks in the steam flow, drew out a small amount of seed and H2O2 with a plastic pipette and let 2 or 3 drops into the flask. Sealed the flask again and let the seed/H2O2 mixture spread over the agar.

I then put the flasks under a bright light to get the H2O2 to break down into water and hydrogen ( and stop acting as a bleach). After that the flasks were kept on a windowsill at about 22-25C.

Those are the basics but I did sterilize everything I used, including the outside of the flasks, with H2O2. I also wore rubber gloves and used alcohol gel on them in an attempt to keep things sterile. So far so good :D

EDITED several months later. This method did not work well.

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Last edited by cricketerry on Fri Dec 31, 2010 10:12 pm, edited 1 time in total.

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PostPosted: Wed Jun 10, 2009 8:49 am 
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cricketerry wrote:
I then put the flasks under a bright light to get the H2O2 to break down into water and hydrogen ( and stop acting as a bleach). After that the flasks were kept on a windowsill at about 22-25C.


Well, that's something I'm not doing so maybe that is the trick. I would expect the Mn in the medium to quickly break down the H2O2... Maybe I'm wrong.

tt

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PostPosted: Thu Jun 11, 2009 9:17 am 
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so happy for you Terry! keep us updated please :)

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